Sanger Sequencing

Title Description Accession Platform Publication
Picea glauca cDNA clone Cortical resin ducts were dissected using a laser microdissection system from methyl jasmonate-induced 2-year-old white spruce PG-653 seedlings. GW726474GW732338 Sanger Sequencing Celedon et al 2017
Picea glauca cDNA clone Cambium tissue was dissected using a laser microdissection system from methyl jasmonate-induced 2-year-old white spruce PG-653 seedlings. GW732339GW738147 Sanger Sequencing Celedon et al 2017

RNA-Seq Experiment

Title Description Accession Platform Publication
Picea glauca PG-653 LMD Differential expression analysis Cell-types (cortical resin ducts (CRD) epithelial cells, cortex polyphenolic (CP) cells and phloem (Phlo) cells) were isolated by laser microdissection from bark tissue in white spruce trees treated with methyl jasmonate or tween (control). RNA extraction and RNA sequencing was performed with isolated cell-types for transcriptome analysis. Gene expression and annotation of transcripts was done to study the defense response in white spruce trees at the cell-type specific level. PRJNA290034 Illumina
Hi-Seq
Celedon et al 2017
Picea glauca shoot development time course Shoots were sampled over 4 weeks during shoot development. Four individual clones were sampled each week. The goal of the experiment was to study the changes in gene expression over time during shoot development. PRJNA309861 Illumina
Hi-Seq
Mageroy et al 2017